Application Data

2L-ChiralTLC分離条件検討例

2L-ChiralTLC IA

Warfarin Sodium N-CBZ-DL-Valine Flavanone
Warfarin Sodium

Plate size: 10mm×100mm
Solvent:n-Hex./IPA/TFA=50/50/0.1(v/v/v)
Sample conc.: 50,000ppm in Eluent
Inj. : 1µL.
Detect:UV254nm

N-CBZ-DL-Valine

Plate size: 10mm×100mm
Solvent:n-Hex./IPA/TFA=70/30/0.1(v/v/v)
Sample conc.: 100,000ppm in Eluent
Inj. : 1µL.
Detect:Phosphomolybdic acid

Flavanone

Plate size: 10mm×100mm
Solvent:n-Hex./EtOAc=90/10(v/v)
Sample conc.: 5,000ppm in Eluent
Inj. : 1µL.
Detect:UV254nm

2L-ChiralTLC IC

4-Benzoyloxy-2-azetidinone Pindolol 1,5-dihydroxy-1,2,3,4-tetrahydronaphthalene
4-Benzoyloxy-2-azetidinone

Plate size: 10mm×100mm
Solvent:n-Hex./IPA=50/50(v/v)
Sample conc.: 100,000ppm in Eluent
Inj. : 1µL.
Detect:UV254nm

Pindolol

Plate size: 10mm×100mm
Solvent:n-Hex./IPA/DEA=70/30/0.1(v/v/v)
Sample conc.: 10,000ppm in Eluent
Inj. : 1µL.
Detect:UV254nm

1,5-dihydroxy-1,2,3,4-tetrahydronaphthalene

Plate size: 10mm×100mm
Solvent:DCM/MeOH/AcOH=100/0.1/0.1(v/v/v)
Sample conc.: 50,000ppm in Eluent
Inj. : 1µL.
Detect:Iodine

2L-ChiralTLC ID

Flavanone Aminoglutethimide
Flavanone

Plate size: 10mm×100mm
Solvent:MeOH=100(v)
Sample conc.: 5,000ppm in Eluent
Inj. : 1µL.
Detect:UV254nm

Aminoglutethimide

Plate size: 10mm×100mm
Solvent:EtOAc/DEA=100/0.1(v/v)
Sample conc.: 50,000ppm in Eluent
Inj. : 1µL.
Detect:UV254nm

2L-ChiralTLC IF

Tröger's base Furoin Methyclothiazide
Tröger's base

Plate size: 10mm×100mm
Solvent:MeOH=100(v)
Sample conc.: 10,000ppm in Eluent
Inj. : 1µL.
Detect:UV254nm

Furoin

Plate size: 10mm×100mm
Solvent:n-Hex./EtOH/MeOH=30/40/40(v/v/v)
Sample conc.: 50,000ppm in Eluent
Inj. : 1µL.
Detect:UV254nm

Methyclothiazide

Plate size: 10mm×100mm
Solvent:n-Hex./EtOH/DEA=50/50/0.1(v/v)
Sample conc.: 10,000ppm in Eluent
Inj. : 1µL.
Detect:UV254nm

※ n-Hexane=n-ヘキサン, MeOH=メタノール, EtOH=エタノール, DCM=ジクロロメタン, EtOAc=酢酸エチル, IPA=2-プロパノール, DEA=ジエチルアミン, AcOH=酢酸, TFA=トリフルオロ酢酸